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KMID : 1023420070070010021
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Journal of Dental Hygiene Science
2007 Volume.7 No. 1 p.21 ~ p.24
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Analysis of Acid Stress Response in Streptococcus mutans KCTC 3065
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Kang Kyung-Hee
Kim Ji-Young
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Abstract
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Dental caries is initiated by the acid accumulated in dental plaque. Streptococcus mutans, one of a major causal agents of dental caries, is component of the dental plaque and produces various organic acids such as lactic acid as the end-product of glycolysis. As a consequence, we investigated the acid stress response of S. mutans KCTC 3065 in this study. The addition of lactic acid to the growth media had a concentration-dependent effect on the growth of S. mutans. S. mutans exhibited higher maximum culture OD compared with the more acidic growth pH values. At treatment of centration of 20 mmol/L lactic acid in the mid-log phage, cell growth was reduced to 40% relative to the control. The following results were obtained with the treatment of cells with a concentration of 20 mmol/L lactic acid in the mid-log phage for 2hrs: Analysis of fatty acids extracted from cells showed that growth at a concentration of 20 mmol/L lactic acid resulted in changes in C , C, C and C fatty acids. Protein profiles investigated by SDS-PAGE showed that 14:016:018:018:1 approximately 70, 60, 45, 40 and 23 kDa proteins were highly expressed in S. mutans KCTC 3065.
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KEYWORD
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Streptococcus mutans, Acid stress, Growth
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